Production of Taxol by Pestalotiopsis Breviseta Cr01 Isolated from the Catharanceus Roceus and Its Growth Studies. Gowri Karthik, Kathiravangovindarajan and Sukumaran Natarajapillai
نویسنده
چکیده
As there is a continuous search for the alternative source for taxol, a million dollar drug due to its scarcity scientists have chosen microorganism as one of the alternate sources.Pestalotiopsis breviseta CR01 produces taxol invitro which was isolated from the plant Catharanceus roceus. The optimum growth of this fungus was analyzed by varying different growth parameters. The presence of taxol in this fungus was confirmed through HPTLC, UV and IR spectroscopic methods. The amount of taxol was quantified through HPLC analysis. This study reveals that Pestalotiopsis brevisetaCR01 optimum growth can be attained at temperature 25°C and pH 7.5 with the presence of dextrose as carbon source.
منابع مشابه
Isolation of anticancer drug TAXOL from Pestalotiopsis breviseta with apoptosis and B-Cell lymphoma protein docking studies
BACKGROUND Extraction and investigation of TAXOL from Pestalotiopsis breviseta (Sacc.) using protein docking, which is a computational technique that samples conformations of small molecules in protein-binding sites. Scoring functions are used to assess which of these conformations best complements the protein binding site and active site prediction. MATERIALS AND METHODS Coelomycetous fungi ...
متن کاملA Review on Taxol Production through Biotechnological Approaches
Taxol is a very important anticancer drug which was first isolated from Yew plant (Taxus spp.). However, Taxol supply by extraction from natural sources, has several limitations, and cannot meet current market's demands. Therefore, it seems necessary to use alternative production methods. Producing Taxol through biotechnological approaches is among the main options which have some advantages su...
متن کاملEffect of taxol from Pestalotiopsis mangiferae on A549 cells-In vitro study
Pestalotiopsis mangiferae Coelomycete fungi were used to examine the production of taxol. The taxol isolated from this fungus is biologically active against cancer cell lines were investigated for its antiproliferative activity in human Non Small Cell Lung Cancer A549 cells. The results showed that the methylene chloride extraction of Pestalotiopsis mangiferae inhibited the proliferation of A 5...
متن کاملOver - expression Effect of Gene Encoding 3-hydroxy-3-Methylglutaryl-CoA Reductase on Production of Taxol in Iranian Hazel (Corylus avellana L.)
Background: Sustainable and commercial production of taxol as an anti cancer drug is a critical point to its clinical application. Nowadays, hazel because of rapid growth and wide range distribution is considered as an alternative source of Taxol. Objective: To increase taxol production the cDNA encoding 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) from Iranian hazel (GeneBank accession num...
متن کاملHazel (Corylus avellana L.) as a New Source of Taxol and Taxanes
Taxol is used to treatment of variety cancers including ovarian, breast, lung, head and neck carcinomas and the AIDS-related Karposi’s carcinoma, and was originally obtained from bark of taxus brevifolia. However the amount of extracted taxol from taxus is extremely low and cannot be considered as a suitable commercial source and it has led to studies for finding new sources of Taxol. In 1998, ...
متن کامل